Role of cytokine receptor-like factor 1 in hepatic stellate cells and fibrosis

Figure 3 Overexpression of cytokine receptor-like factor 1 in normal liver does not increase expression of profibrotic cytokines. A: Overexpression of cytokine receptor-like factor 1 (CRLF1). Control adenovirus was injected into tail vein of two mice and adenovirus expressing cardiotrophin-like cytokine factor 1 (CLCF1) gene was injected into three mice. After three days liver proteins and RNA were extracted and expression of CLRF1 protein was analyzed by Western blot (top panel) and CLRF1 mRNA was analyzed by reverse transcription-polymerase chain reaction (RT-PCR) (bottom panel); B: Expression of profibrotic cytokines. Expression of the indicated profibrotic cytokines was analyzed by RT-PCR in the control (CON) and CRLF1 overexpressing livers. PROT: Protein; CNTFR: Ciliary neurotrophic factor receptor; IL: Interleukin; TNF-α: Tumour necrosis factor-α; KC: Chemokine.