Fucosylation and gastrointestinal cancer

Figure 3 Deficiency of GMDS leads to escape from NK cell-mediated tumor surveillance through modulation of TRAIL signaling[109]. A: After trasnfection of the wild-type GMDS gene into HCT116 cells, Western blot analysis of GMDS and AAL blot analysis were performed. The binding to AAL was restored in transfected cells (WT-GMDS); B: Tumor growth of the GMDS-rescued cells on the backs of athymic nude mice was significantly suppressed compared to mock cells. The bar indicates 10 mm; C: When athymic nude mice were treated with anti-asialo GM1 antibody to deplete NK cells, the tumor growth of the GMDS-rescued cells was accelerated, but not in the case of mock cells. D: The higher susceptibility of the GMDS-rescued cells to TRAIL was confirmed by clonogenic survival assays. These figures are modified from the data in reference 109.