Suppression of the STAT3/MYC/MCL-1 pathway by the fixed Jianpi Huoxue Formula relieves early biochemical abnormalities in liver fibrosis

Figure 2 Single-cell RNA sequencing of liver tissues from cirrhotic patients. Six liver samples, divided into the control group (n = 3) and the Jianpi Huoxue Formula (JHF) group (n = 3), were analyzed using single-cell RNA sequencing. A: Diagram showing the experimental procedure in this study; B: Fourteen major liver cell types were identified based on cell-type-specific marker genes after JHF treatment; C: Gene Ontology and terms and Kyoto Encyclopedia of Genes and Genomes analyses of the top 20 differentially expressed genes in human hepatic stellate cells (HSCs) subtypes; D: Violin plots and Uniform Manifold Approximation and Projection (UMAP) plots showing the expression levels of STAT3, MYC, and MCL-1 across HSC subpopulations after JHF treatment; E: Violin plots and UMAP plots showing the expression levels of ACTA2, COL1A1, COL1A2, and MMP1 in the major cell population.