Methionine depletion inhibits hepatocellular carcinoma by inducing ferroptosis and suppressing epithelial-mesenchymal transition through the ARNT2/UBE2Z pathway

Figure 5 Silencing aryl hydrocarbon receptor nuclear translocator 2 inhibits epithelial-mesenchymal transition and promotes ferroptosis in hepatocellular carcinoma cells. A: ACSL4 and GPX4 expression levels were detected by Western blot; B: Intracellular lipid peroxidation was assessed by malondialdehyde assay kit; C: Intracellular reactive oxygen species contents were determined by the fluorescent probe DCFH-DA; D: Intracellular glutathione levels were determined using the glutathione colorimetric assay; E: E-cadherin and N-cadherin contents were determined with Western blot; F: Cell proliferation activity was evaluated by the CCK-8 assay; G: Cell invasion was evaluated by Transwell assay. Results indicate mean ± SD, n = 3. ^bP < 0.01; ^cP < 0.001. MDA: Malondialdehyde; GSH: Glutathione.