Methionine depletion inhibits hepatocellular carcinoma by inducing ferroptosis and suppressing epithelial-mesenchymal transition through the ARNT2/UBE2Z pathway

Figure 4 Methionine regulates the expression of ARNT2, which is associated with hepatocellular carcinoma status and poor prognosis. A: ARNT2 levels in hepatocellular carcinoma cells were measured by Western blot; B: ARNT2 levels in liver hepatocellular carcinoma were analyzed using the GEPIA 2 online tool; C-G: ARNT2 expression in LIHC under different clinical conditions was assessed using the ULCAN online tool; H-J: The association between ARNT2 expression and survival was analyzed using the Kaplan-Meier online tool; K: Survival analysis of ARNT2 expression was conducted using the GEPIA 2 online tool; L: ARNT2 expression was evaluated by immunohistochemical staining. Results are presented as the mean ± SD, n = 3. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. LIHC: Liver hepatocellular carcinoma; HCC: Hepatocellular carcinoma; Met-: Methionine-deficient; Met+: Methionine-supplemented (100 μm); NS: Not significant.