Methionine depletion inhibits hepatocellular carcinoma by inducing ferroptosis and suppressing epithelial-mesenchymal transition through the ARNT2/UBE2Z pathway

Figure 3 Methionine supplementation promotes epithelial-mesenchymal transition, while methionine deficiency inhibits epithelial-mesenchymal transition in hepatocellular carcinoma cells. A: E-cadherin and N-cadherin levels were detected by Western blot; B: Cell proliferation was assessed using the clone formation assay; C: Cell migration was evaluated using the scratch healing assay; D: Cell invasion was assessed using the Transwell assay. Results are presented as the mean ± SD, n = 3. ^aP < 0.05; ^bP < 0.01; ^cP < 0.001. Met-: Methionine-deficient; Met+: Methionine-supplemented (100 μm).