Differential impact of high-fructose and ethanol diets on early steatohepatitis and hepatic melanocortin-4 receptor responses in rats

Figure 7 Indirect immunofluorescence staining for the proliferation marker Ki-67. A: Representative liver sections show Ki-67-positive nuclei at week 4. DAPI was used for nuclear counterstaining (× 20); B: Indices of hepatocellular DNA synthesis were quantified as the percentage of Ki-67-positive cells across eight high-power fields per liver sample. Statistical analysis was performed using one-way ANOVA. Data are expressed as mean ± SEM (n = 5/group). ^aP < 0.05 vs control, ^bP < 0.05 vs HFD. HFD: High-fat diet; HF-EFr: High-fat plus ethanol and fructose; HFrD: High-fructose diet.