Plasma exosomal miR-137 activates natural killer cells in primary biliary cholangitis by targeting NFATC1

Figure 6 Overexpression plasmid mimics of microRNA-137 (miR-137), miR-124 and miR-506. A: Showed the level of perforin secretion after transfection with microRNA-124 (miR-124), miR-137 and miR-506 mimics compared to negative control (NC). Overexpression of the three miRNAs had no significant effect on perforin (P > 0.05); B: Showed the level of perforin secretion after transfection with miR-124, miR-137, and miR-506 mimics compared to the control. The miR-137 (P < 0.05) overexpression increased granzyme B production by natural killer cells, whereas miR-124 (P > 0.05) and miR-506 (P > 0.05) had no significant effect on granzyme B; C: Showed the level of interferon gamma (IFN-γ) secretion after transfection with miR-124, miR-137, and miR-506 mimics compared to NC. Overexpression of the three miRNAs had no significant effect on IFN-γ (P > 0.05); D: Showed the level of tumor necrosis factor alpha (TNF-α) secretion after transfection with miR-124, miR-137, and miR-506 mimics compared to NC. Overexpression of the three miRNAs had no significant effect on TNF-α (P > 0.05).