Novel nomogram for differential diagnosis of UGT1A1 gene mutation-associated unconjugated hyperbilirubinemia with hemolytic diseases

Table 2 Determination of optimal diagnostic cutoff values for variables selected by least absolute shrinkage and selection operator regression using receiver operating characteristic curve analysis

	AUC (95%CI)	Accuracy (95%CI)	Sensitivity (95%CI)	Specificity (95%CI)	PPV (95%CI)	NPV (95%CI)	Cut off
HCT	0.903 (0.839-0.966)	0.826 (0.776-0.867)	0.872 (0.748-0.940)	0.817 (0.760-0.862)	0.506 (0.400-0.612)	0.967 (0.931-0.985)	40.152
RDWSD	0.953 (0.910-0.997)	0.955 (0.923-0.974)	0.872 (0.748-0.940)	0.972 (0.941-0.987)	0.872 (0.748-0.940)	0.972 (0.941-0.987)	45.55
RET percentage	0.986 (0.971-1.00)	0.936 (0.899-0.962)	0.957 (0.858-0.988)	0.931 (0.890-0.958)	0.750 (0.628-0.842)	0.990 (0.965- 0.997)	2.095
IBiL/TBiL ratio	0.776 (0.673-0.857)	0.830 (0.780-0.871)	0.660 (0.517-0.778)	0.867 (0.815- 0.906)	0.517 (0.393-0.638)	0.922 (0.877-0.951)	0.776

AUC: Area under the receiver operating characteristic; CI: Confidence interval; PPV: Positive predictive value; NPV: Negative predictive value; HCT: Hematocrit; RDWSD: Red cell distribution width-standard deviation; RET: Reticulocyte; IBiL: Indirect bilirubin; TBiL: Total bilirubin.