Qushi Huoxue ointment ameliorates metabolic associated steatotic liver disease through autophagy activation and ferroptosis inhibition

Figure 5 Qushi Huoxue ointment activates hepatic autophagy in metabolic associated steatotic liver disease mice. A: Representative western blot images of Beclin1, the light chain 3 II/Light chain 3 I, and P62 protein levels in liver tissues; B: Quantitative analysis of Beclin1, the light chain 3 II/Light chain 3 I ratio, and P62 protein levels; C: Quantitative reverse-transcription polymerase chain reaction analysis of mRNA expressions of Beclin1 and P62 in liver tissues. Data are presented as means ± SD; ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. LC3II/LC3I: Light chain 3 II/Light chain 3 I ratio; MASLD: Metabolic associated steatotic liver disease; QSHXO: Qushi Huoxue ointment.