Transforming growth factor beta reduces proprotein convertase subtilisin/kexin type 9 in the supernatant of hepatic stellate cells

Figure 2 Effect of lipopolysaccharide and transforming growth factor beta on proprotein convertase subtilisin/kexin type 9 in the culture medium of LX-2 cells. A: Proprotein convertase subtilisin/kexin type 9 (PCSK9) in the LX-2 cell culture medium after 24 hours of cultivation with 0 μg/mL, 1 μg/mL, or 10 μg/mL lipopolysaccharide (4 independent experiments); B: PCSK9 in the LX-2 cell culture medium after 24 hours of cultivation with 0 ng/mL, 5 ng/mL, or 7 ng/mL transforming growth factor beta (TGF-β; 6 independent experiments for controls and 7 ng TGF-β, and 3 independent measures for 5 ng/mL); C: PCSK9 protein of LX-2 cells cultivated with 0 ng/mL, 5 ng/mL, or 7 ng/mL TGF-β for 24 hours. PCSK9: Proprotein convertase subtilisin/kexin type 9; TGF-β: Transforming growth factor beta; GAPDH: Glyceraldehyde-3-phosphate dehydrogenase.