Bile acids in metabolic dysfunction-associated steatotic liver disease

Figure 1 Two major bile acid biosynthetic pathways are shown. In the classic (or neutral) pathway, cholesterol is converted to 7α cholesterol by the rate-limiting enzyme cholesterol 7α-hydroxylase (cytochrome P450 family 7 subfamily A member 1), which is located in the endoplasmic reticulum. The alternative (or acidic) pathway is initiated by cytochrome P450 family 27 subfamily A member 1 (CYP27A1) in mitochondria. Cytochrome P450 family 7 subfamily B member 1 (CYP8B1) is required to synthesize cholic acid (CA). CYP7B1 is required to synthesize chenodeoxycholic acid (CDCA) in the acidic pathway. The neutral pathway is also able to form CDCA by CYP27A1. Primary bile acids (BAs) CA and CDCA are metabolized by intestinal bacteria to form the secondary BAs, deoxycholic acid and lithocholic acid. CA: Cholic acid; CDCA: Chenodeoxycholic acid; CYP27A1: Cytochrome P450 family 27 subfamily A member 1; CYP7A1: Cytochrome P450 family 7 subfamily A member 1; CYP8B1: Cytochrome P450 family 8 subfamily B member 1; DCA: Deoxycholic acid; LCA: Lithocholic acid.