Hepatitis B virus rtCYE/rtCYEI mutations may contribute limited tenofovir resistance: Analysis of a large sample of Chinese patients

Table 4 Drug susceptibility analysis of representative hepatitis B virus strains, mean ± SD

Viral strain	Tenofovir disoproxil fumarate
	EC50 (μmol/L)2	Fold3	P value1
rtWT (wild-type)	5.79 ± 1.59	1.0	-
rtWT (laboratory)	5.90 ± 1.95	1.0	0.93
rtCYE	7.41 ± 0.97	1.3	0.09
rtCYEI	8.51 ± 1.29	1.5	< 0.05
rtCYE+M204I	6.95 ± 0.64	1.2	0.17
rtCYE+L80I+L180M+M204I	6.92 ± 1.69	1.2	0.31
rtCYEI+M204I	8.92 ± 0.87	1.5	< 0.05
rtCYEI+L80I+L180M+M204I	10.23 ± 1.01	1.8	< 0.05
rtCYEI+A181V	36.15 ± 5.99	6.2	< 0.05
rtCYEI+N236T	57.33 ± 5.30	9.9	< 0.05

¹Comparison with rtWT.

²EC₅₀: Half maximal effective concentration.

³Fold: The EC₅₀ of mutant/the EC₅₀ of wild-type. Experiments were performed at least three times independently.

WT: Wild-type; rtCYE: rtS106C+H126Y+D134E; rtCYEI: rtS106C+H126Y+D134E+L269I.