Tumor necrosis factor alpha-induced protein 3: A key biomarker for response to ursodeoxycholic acid in primary biliary cholangitis

Figure 3 Identification of common differentially expressed genes and investigation of tumor necrosis factor alpha-induced protein 3 expression in primary biliary cholangitis. A: Venn diagram illustrated the common differentially expressed genes among three datasets (GSE159676, GSE93170, and GSE119600). A total of 13 differentially expressed genes were identified to be shared across all three datasets, including MYOF, NEDD9, CELF2, SSH2, SEMA4D, tumor necrosis factor alpha-induced protein 3 (TNFAIP3), TAGAP, BTLA, ARRDC2, FAHD1, PNP, SUMO1, and UTY; B: Immunohistochemistry of TNFAIP3 expression in the liver tissues from healthy controls (HCs) and primary biliary cholangitis (PBC) patients. Representative images showed the distinct staining pattern in both groups. Scale bars: 100 μm (upper panel), 20 μm (lower panel); C: Quantification of TNFAIP3 expression in the liver tissue from HCs and PBC patients. The relative TNFAIP3 expression level in the liver tissue of PBC was significantly higher than in HCs (^aP < 0.0001). PBC: Primary biliary cholangitis; HCs: Healthy controls; TNFAIP3: Tumor necrosis factor alpha-induced protein 3.