Integrated serum metabolomics reveal molecular mechanism of Xietu Hemu prescription on metabolic dysfunction-associated steatotic liver disease-related obesity

Figure 2 RNA sequencing analysis of the differences in 3T3-L1 cell differentiation. A: Schematic diagram of 3T3-L1 cell culture and differentiation process; B: Oil red O staining images in the control group vs model group on day 6; C: Box plot of gene expression of samples (n = 3); D: Principal coordinate analysis plot of gene expression of samples (n = 3); E: The number of up-regulated or down-regulated differential genes between control and model groups (n = 3); F and G: Heatmap (F) and volcano plot (G) of differential gene expression for genes demonstrating significant differences based on the screening criteria of |Log2FC| ≥ 2 and q value ≤ 0.05 (n = 3); H-N: Comparison expression of key genes for lipid droplet accumulation and differentiation in control and model groups. Data were expressed as mean ± SEM. 400 ×, 25 μm. ^aP < 0.05 vs control; ^bP < 0.005 vs control; ^cP < 0.001 vs control. PCoA: Principal coordinate analysis; NCS: Newborn calf serum; FBS: Fetal bovine serum; MDI: 3-isobutyl-1-methylxanthine, dexamethasone, and insulin; TPM: Transcripts per million.