Yinhuang granule alleviates carbon tetrachloride-induced liver fibrosis in mice and its mechanism

Figure 3 Yinhuang granule decreased hepatic stellate cells activation in carbon tetrachloride-treated mice. A: Hepatic mRNA expression of Col1a1, Col3a1 and Fn1 (n = 5-6); B: Hepatic mRNA expression of Tgfb1 [transforming growth factor (TGF)-β] and Acta2 [α-smooth muscle actin (α-SMA)] (n = 6); C: The expression of liver α-SMA protein was detected by Western blot, and β-actin was used as a loading control. The results represent four independent experiments; D: The protein bands of α-SMA were normalized to basal β-actin expression (n = 4). Data were expressed as mean ± SEM. ^aP < 0.05, ^bP < 0.01 vs control vehicle; ^cP < 0.05, ^dP < 0.01 vs carbon tetrachloride vehicle. CCl₄: Carbon tetrachloride; YHG: Yinhuang granule.