Yinhuang granule alleviates carbon tetrachloride-induced liver fibrosis in mice and its mechanism

Table 1 List of Reagents

Reagents	Company
Anti-Lamin B1	Hangzhou Hua-An Biotechnology Co., Ltd. (Hangzhou, China)
Anti-α-SMA	Cell Signaling Technology (Danvers, MA, United States)
Anti-NRF1	Cell Signaling Technology (Danvers, MA, United States)
Anti-β-actin	Cell Signaling Technology (Danvers, MA, United States)
Anti-Nrf2	Gene Tex Inc. (Alton Parkway lrvine, CA, United States)
Anti-GCLC	Santa Cruz (Santa Cruz, CA, United States)
Anti-NQO1	Abways Technology, Inc. (Shanghai, China)
Anti-GCLM	Abways Technology, Inc. (Shanghai, China)
Anti-PGC1	Abcam (Shanghai, China)
Kits for detecting ALT/AST activity	Nanjing Jiancheng Bioengineering Institute (Nanjing, China)
Kits for detecting liver hydroxyproline content	Nanjing Jiancheng Bioengineering Institute (Nanjing, China)
Kits for detecting hepatic GSH	Nanjing Jiancheng Bioengineering Institute (Nanjing, China)
Kits for detecting protein carbonylation amount	Nanjing Jiancheng Bioengineering Institute (Nanjing, China)
Kits for detecting SOD activity	Nanjing Jiancheng Bioengineering Institute (Nanjing, China)
ATP content	Beyotime Biotech (Shanghai, China)
ELISA kits	RapidBio (West Hills, CA, United States)
mitochondrial DNA extraction	Sangon Biotech (Shanghai, China)
NE-PER nuclear and cytoplasmic extraction reagents	Thermo Fisher Scientific (Waltham, MA, United States)
BCA Protein Assay Kits	Thermo Fisher Scientific (Waltham, MA, United States)
PrimeScript Master Mix	Takara (Shiga, Japan)
SYBR Premix Ex Taq	Takara (Shiga, Japan)
Trizol reagent	Life Technology (Carlsbad, CA, United States)
2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA)	Life Technology (Carlsbad, CA, United States)

Other reagents unless indicated were obtained from Sigma Chemical Co. (St. Louis, MO, United States).