Hepatitis B virus markers in hepatitis B surface antigen negative patients with pancreatic cancer: Two case reports

Figure 1 Immunohistochemistry of resected pancreatic tissues. Case 1 and case 2 are subjects with pancreatic ductal adenocarcinoma and positive markers of hepatitis B virus (HBV) infection. Control refers to pancreatic tissue of a patient with pancreatic cancer, negative for markers of current and previous HBV infection (control case is not described). Samples were stained for Ki-67 protein (green fluorescence) and HBV regulatory X protein (HBx) (red fluorescence). Cell nuclei were counterstained with Hoechst33342 dye (blue). A, C and E: Images at magnification 10 ×; B, D and F: Images at magnification 100 ×. Arrows indicate HBx/Ki-67 co-stained cells. Median Ki-67 index (%): Subject 1 - 77, Subject 2 - 68, Сontrol - 55.