Adult human liver slice cultures: Modelling of liver fibrosis and evaluation of new anti-fibrotic drugs

doi:10.4254/wjh.v13.i2.187

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Feb 27, 2021 (publication date) through Apr 25, 2024

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World Journal of Hepatology

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1948-5182

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Hepatol. Feb 27, 2021; 13(2): 187-217
Published online Feb 27, 2021. doi: 10.4254/wjh.v13.i2.187

Table 2 Primers used for real-time reverse transcription-quantitative polymerase chain reaction analysis

Gene	Forward primer sequence	Reverse primer sequence
CYP2E1	AGCACAAACTCTGAGATATGG	ATAGTCACTGTACTTGAACT
CYP3A4	GCCTGGTGCTCCTCTATCTA	ACAGGCTGTTGACCATCATAAAAG
HNF-1β	ACGTCAGAAAGCAACGAGAGATC	CCCAGGCCCATGGCT
HNF-4α	CCTGGAATTTGAGAATGTGCAG	AGGTTGGTGCCTTCTGATGG
Albumin	ATGAGATGCCTGCTGACTTG	GCACGACAGAGTAATCAGGA
18S RNA	CAGAGCGAAAGCATTTGCCAAG	CGGCATCGTTTATGGTCGGAAC
TGF-β1	CCTGGAAAGGGCTCAACAC	CAGTTCTTCTCTGTGGAGCTGA
HSP 47	GCCACCGTGGTGCCGCA	GCCAGGGCCGCCTCCAGGAG
β-SMA	AGGGGGTGATGGGTGGGAA	ATGATGCCATGTTCTATCGG
Procol1A1	CAATCACCTGCGTACAGAACGCC	CGGCAGGGCTCGGGTTTC
MMP-2	CTT CGCCCC AGG CAC TGG TG	CCTCGCTCCCATGGG GTT CGGT
MMP-9	GGT CCCCCCACT GCT GGC CCTTCTACGGCC	GTCCTCAGG GCACTG GAG GAT GTC ATA GCT
VEGF	TACCTCCACCATGCCAAGTG	ATGATTCTGCCCTCCTCCTTC
GAPDH	ACCAGGGCTGCTTTTAACTCT	GGTGCCATGGAATTTGCC

Citation: Kartasheva-Ebertz D, Gaston J, Lair-Mehiri L, Massault PP, Scatton O, Vaillant JC, Morozov VA, Pol S, Lagaye S. Adult human liver slice cultures: Modelling of liver fibrosis and evaluation of new anti-fibrotic drugs. World J Hepatol 2021; 13(2): 187-217
URL: https://www.wjgnet.com/1948-5182/full/v13/i2/187.htm
DOI: https://dx.doi.org/10.4254/wjh.v13.i2.187