High omega arachidonic acid/docosahexaenoic acid ratio induces mitochondrial dysfunction and altered lipid metabolism in human hepatoma cells

Figure 3 Effect of arachidonic acid: docosahexaenoic acid ratios on mechanistic mediators of lipid metabolism. A-E: VL17-A cells were treated with different ratios of arachidonic acid: docosahexaenoic acid for 24 h. Following this, the expression of several proteins that modulate lipid metabolism were examined, namely, SCD1 (A), PPAR-α (B), SREBP1c (C), CB1 (D) and CB2 (E). ^aP ≤ 0.05 and ^bP ≤ 0.01 compared to control; ^cP < 0.05 and ^dP < 0.01 compared to 1:1 ratio; ^eP < 0.05 compared to 4:1 ratio. Data is presented as mean ± SE (n = 3-4). AA: Arachidonic acid; DHA: Docosahexaenoic acid.