High omega arachidonic acid/docosahexaenoic acid ratio induces mitochondrial dysfunction and altered lipid metabolism in human hepatoma cells

Figure 2 Effect of arachidonic acid: docosahexaenoic acid ratios on intracellular triglyceride accumulation. A-C: VL17-A cells were treated with different ratios of arachidonic acid: docosahexaenoic acid for 24 h (A), 48 h (B) and 72 h (C). Intracellular triglyceride levels were measured and normalised to cellular protein content. ^aP < 0.05 and ^bP ≤ 0.01 compared to control; ^cP < 0.05 and ^dP ≤ 0.01 compared to 1:1 ratio. Data is presented as mean ± SE (n = 5). AA: Arachidonic acid; DHA: Docosahexaenoic acid.