Prolonged high-fat-diet feeding promotes non-alcoholic fatty liver disease and alters gut microbiota in mice

Figure 3 Histological assessment of the liver tissue in mice subjected to prolonged high-fat diet-feeding. A: Hematoxylin and eosin; B: Picrossirious red; C: Oil red O images of liver tissue (200 X); D: Histological score of microvesicular steatosis; E: Microvesicular steatosis; F: Ballooing degeneration of hepatocytes; G: Acidophilic bodies; H: Lobular inflammation; I: Fibrosis; J: Non-alcoholic steatohepatitis score. Data are expressed as median with interquartile range. n = 6-11 mice per group. ^aSignificantly different from Young-LFD (P < 0.05), ^bSignificantly different from Old-LFD (P < 0.05). LFD: Low-fat diet.