Comprehensive analysis of HFE gene in hereditary hemochromatosis and in diseases associated with acquired iron overload

Table 4 Linkage disequilibrium between HLA-B alleles and HFE coding region IVS2(+4)T>C single nucleotide polymorphism alleles

	Observed frequency		Expected frequency		Standardized value of disequilibrium(D’)		Standardized value of correlation(r2)	QUI2value	P value of QUI2
HLA-B	HFE IVS2(+4)T>C
	T	C	T	C	T	C
07	13	5	10.98	7.02	0.2877	-0.2877	0.0052	1.0471	0.3062
08	8	5	7.93	5.07	0.0138	-0.0138	0.0000	0.0017	0.9672
13	4	0	2.44	1.56	1.0000	-1.0000	0.0130	2.6096	0.1062
14	4	6	6.10	3.90	-0.3443	0.3443	0.0098	1.9513	0.1624
15	11	6	10.37	6.63	0.0950	-0.0950	0.0005	0.1073	0.7433
18	8	2	6.10	3.90	0.4872	-0.4872	0.0080	1.5973	0.2063
27	1	3	2.44	1.56	-0.5902	0.5902	0.0111	2.2235	0.1359
35	17	4	12.81	8.19	0.5116	-0.5116	0.0196	3.9264	0.0475
37	0	3	1.83	1.17	-1.0000	1.0000	0.0238	4.7638	0.0291
38	2	1	1.83	1.17	0.1453	-0.1453	0.0002	0.0411	0.8393
39	1	5	3.66	2.34	-0.7268	0.7268	0.0256	5.1103	0.0238
40	3	0	1.83	1.17	1.0000	-1.0000	0.0097	1.9472	0.1629
41	2	1	1.83	1.17	0.1453	-0.1453	0.0002	0.0411	0.8393
42	1	0	0.61	0.39	1.0000	-1.0000	0.0032	0.6426	0.4228
44	6	13	11.59	7.41	-0.4823	0.4823	0.03821	7.6388	0.0057
45	3	3	3.66	2.34	-0.1803	0.1803	0.0016	0.3146	0.5749
48	2	0	1.22	0.78	1.0000	-1.0000	0.0065	1.2916	0.2558
49	8	0	4.88	3.12	1.0000	-1.0000	0.0266	5.3279	0.0210
50	2	0	1.22	0.78	1.0000	-1.0000	0.0065	1.2916	0.2558
51	10	4	8.54	5.46	0.2674	-0.2674	0.0034	0.6882	0.4068
52	3	4	4.27	2.73	-0.2974	0.2974	0.0050	1.0037	0.3164
53	5	0	3.05	1.95	1.0000	-1.0000	0.0164	3.2787	0.0702
55	0	2	1.22	0.78	-1.0000	1.0000	0.0158	3.1598	0.0755
56	1	0	0.61	0.39	1.0000	-1.0000	0.0032	0.6426	0.4228
57	4	6	6.10	3.90	-0.3443	0.3443	0.0098	1.9513	0.1624
58	1	5	3.66	2.34	-0.7268	0.7268	0.0256	5.1103	0.0238
67	1	0	0.61	0.39	1.0000	-1.0000	0.0032	0.6426	0.4228

¹The higher value of correlation. Identification of single nucleotide polymorphisms and most frequent allele according to NCBI (http://www. ncbi.nlm.nih.gov) and Ensembl (http://www.ensembl.org): rs2071303 (IVS2(+4)T>C). Shaded cells are showing significant LD values.