Bioengineered humanized livers as better three-dimensional drug testing model system

Figure 2 Xenogenic liver before and after perfusion acellularization. Macroscopic appearance (optical) of acellularized liver showing removal of hematopoietic, liver parenchyma and non-parenchyma cells while retaining the vascular tree. Histological comparison of native and acellularized liver. The expression and distribution of liver extracellular matrix proteins such as collagen type 1 was seen in the parenchymal space as well as around the blood vessels. Fibronectin staining demonstrated conserved meshwork in sinusoidal spaces and biliary ducts post-acellularization. Laminin staining showed intact vasculature throughout the liver scaffold (magnification: 10 ×).