©The Author(s) 2015.
World J Stem Cells. Aug 26, 2015; 7(7): 1064-1077
Published online Aug 26, 2015. doi: 10.4252/wjsc.v7.i7.1064
Published online Aug 26, 2015. doi: 10.4252/wjsc.v7.i7.1064
Table 1 Mouse primer sets used in quantitative real time polymerase chain reaction
| Target-gene | Forward (5’- 3’) | Reverse (5’-3’) | Product size (bp) |
| Oct4 | TCTGTTCCCGTCACTGCTCT | TGTCTACCTCCCTTGCCTTG | 96 |
| Nanog | GCAAGCGGTGGCAGAAAAAC | GCAATGGATGCTGGGATACTCA | 92 |
| Klf4 | AAGCCAAAGAGGGGAAGAAG | CAGTGGTAAGGTTTCTCGCC | 146 |
| Sox2 | CGAACTGGAGAAGGGGAGAG | AAGCGTTAATTTGGATGGGA | 165 |
| Col1 | GCAGGTTCACCTACTCTGTC | CTTGCCCCATTCATTTGTCT | 62 |
| Col2 | ACCCCCAGGTGCTAATGG | AACACCTTTGGGACCATCTTT | 76 |
| Myog | CCTAAAGTGGAGATCCTGCG | GTGGGAGTTGCATTCACTGG | 147 |
| Nestin | AGACAGTGAGGCAGATGAG | CTCTCAGCTGTGGTGGTGAA | 224 |
| Brachyury (T) | CACACCACTGACGCACAC | GAGGCTATGAGGAGGCTTTG | 132 |
| FGF5 | GCTCAATGATCAGAAGGAGGA | TCAGCTGGTCTTGAATGAGG | 175 |
| GATA4 | GATGGGACGGGACACTACCTG | ACCTGCTGGCGTCTTAGATTT | 309 |
| Gapdh | GCACAGTCAAGGCCGAGAAT | GCCTTCTCCATGGTGGTGAA | 151 |
| β-Actin | AGCCATGTACGTAGCCATCC | CTCTCAGCTGTGGTGGTGAA | 228 |
- Citation: McKee C, Perez-Cruet M, Chavez F, Chaudhry GR. Simplified three-dimensional culture system for long-term expansion of embryonic stem cells. World J Stem Cells 2015; 7(7): 1064-1077
- URL: https://www.wjgnet.com/1948-0210/full/v7/i7/1064.htm
- DOI: https://dx.doi.org/10.4252/wjsc.v7.i7.1064