Simplified three-dimensional culture system for long-term expansion of embryonic stem cells

doi:10.4252/wjsc.v7.i7.1064

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Aug 26, 2015 (publication date) through Apr 19, 2024

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Aug 26, 2015; 7(7): 1064-1077
Published online Aug 26, 2015. doi: 10.4252/wjsc.v7.i7.1064

Table 1 Mouse primer sets used in quantitative real time polymerase chain reaction

Target-gene	Forward (5’- 3’)	Reverse (5’-3’)	Product size (bp)
Oct4	TCTGTTCCCGTCACTGCTCT	TGTCTACCTCCCTTGCCTTG	96
Nanog	GCAAGCGGTGGCAGAAAAAC	GCAATGGATGCTGGGATACTCA	92
Klf4	AAGCCAAAGAGGGGAAGAAG	CAGTGGTAAGGTTTCTCGCC	146
Sox2	CGAACTGGAGAAGGGGAGAG	AAGCGTTAATTTGGATGGGA	165
Col1	GCAGGTTCACCTACTCTGTC	CTTGCCCCATTCATTTGTCT	62
Col2	ACCCCCAGGTGCTAATGG	AACACCTTTGGGACCATCTTT	76
Myog	CCTAAAGTGGAGATCCTGCG	GTGGGAGTTGCATTCACTGG	147
Nestin	AGACAGTGAGGCAGATGAG	CTCTCAGCTGTGGTGGTGAA	224
Brachyury (T)	CACACCACTGACGCACAC	GAGGCTATGAGGAGGCTTTG	132
FGF5	GCTCAATGATCAGAAGGAGGA	TCAGCTGGTCTTGAATGAGG	175
GATA4	GATGGGACGGGACACTACCTG	ACCTGCTGGCGTCTTAGATTT	309
Gapdh	GCACAGTCAAGGCCGAGAAT	GCCTTCTCCATGGTGGTGAA	151
β-Actin	AGCCATGTACGTAGCCATCC	CTCTCAGCTGTGGTGGTGAA	228

Citation: McKee C, Perez-Cruet M, Chavez F, Chaudhry GR. Simplified three-dimensional culture system for long-term expansion of embryonic stem cells. World J Stem Cells 2015; 7(7): 1064-1077
URL: https://www.wjgnet.com/1948-0210/full/v7/i7/1064.htm
DOI: https://dx.doi.org/10.4252/wjsc.v7.i7.1064