Mesenchymal stem cells and collagen patches for anterior cruciate ligament repair

Table 1 Real-time polymerase chain reaction primers used for real-time polymerase chain reaction

Abbreviation	Gene name	Forward	Reverse
18S	18S Ribosomal RNA	CGA TGC GGC GGC GTT ATT C	TCT GTC AAT CCT GTC CGT GTC C
HsACAN	Core protein	CAT CAC TGC AGC TGT CAC	AGC AGC ACT ACC TCC TTC
Hs_col1	Collagen 1 A2	GTG GCA GTG ATG GAA GTG	CAC CAG TAA GGC CGT TTG
Hs_col2	Collagen 2 A1	AGCAAGAGCAAGGAGAAG	GGGAGCCAGATTGTCATC
Hs_col3	Collagen 3 A1	GTG AGC CTG GTA AGA ATG G	CCT GGA ACA CCT GGA ATA C
Hs_TNC	Tenascin C	CAC GCT GAG GTT GAT GTT C	GTT GAT GGT CGC TGG ATT G
Hs_SCX	Scleraxis A	CAC CAA CAG CGT GAA CAC	GCA GCG TCT CAA TCT TGG A
Hs_TNMD	Tenomodulin	ACA AGC AAG TGA GGA AGA A	GAC GGC AGT AAA TAC AAC AAT
Hs_MMP13	Collagenase 3	AGT GGT GGT GAT GAA GAT	CTA AGG TGT TAT CGT CAA GTT
Hs_MMP3	Matrix metallopeptidase 3	CAA GGC ATA GAG ACA ACA TAG A	GCA CAG CAA CAG TAG GAT

All primers were run at 61 °C Ta (annealing temperature) and a two-step protocol (95 °C for 10 s and 61 °C for 30 s) and 45 cycles.