Advances in cellular technology in the hematology field: What have we learned so far?

Table 2 Enucleation of Hemangioblasts. The technique here summarized starts by taking the hemangioblasts at the 7^th day of step 2, clarified in Table 1

Step	Procedure
1	Hemangioblasts obtained on day 7 of the procedures described in Table 1 are filtered and plated with Stemline II medium supplemented with inositol, folic acid, monothioglycerol, transferrin, insulin, ferrous nitrate, ferrous sulphate and BSA, along with penicillin-streptomycin solution
2	For 7 d, the medium described in step 1 had hydrocortisone, SCF, interleukin-3 and Epo added; Cell density was kept at 106 cells/mL
3	After the 7th day, SCF and interleukin-3 were no longer added to the medium
4	The cells were co-cultured with human mesenchymal stem cells or OP9 mouse stromal cells at different days between days 19 and 36;
	Medium was composed as described in Step 1 with the addition of Epo

SCF: Stem cell factor; Epo: Erythropoietin; BSA: Bovine serum albumin.