Insulin producing cells established using non-integrated lentiviral vector harboring PDX1 gene

Figure 7 Experiment of pancreatic marker genes consisting of Glut2, PDX-1, Ngn3, somatostatin and glucagon in human adipose tissue derived stem cells^PDX1+ compared to human adipose tissue derived stem cells (negative control) and human pancreatic tissue (positive control) by real time PCR. A: In human adipose tissue derived stem cells (hADSCs), no tested pancreatic markers were amplified except Β-Actin as internal real-time reverse transcription-polymerase chain reaction positive control; B: Expressions of PDX-1 and Ngn3 markers in hADSCs^PDX1+, 7 d after second viral transduction; C: Expressions of PDX1, Ngn3, glucagon, somatostatin and Glut2 markers in hADSCs^PDX1+, 21 d after second viral transduction indicating for beta like cell differentiation; D: Relative gene expression of PDX1, Ngn3, glucagon, somatostatin and Glut2 was mostly increased in hADSCs^PDX1+ compared to human pancreatic tissue on the 21st day after second viral transduction. All PCR products were verified by electrophoresis and sequencing and relative gene expression data were analyzed using 2^-∆∆Ct method.