Nutrient supplemented serum-free medium increases cardiomyogenesis efficiency of human pluripotent stem cells

Table 3 Summary of results: Robustness of SupSB vs SB media for cell lines HES-3, H1 and Siu1-human induced pluripotent stem cells

Conditions		Cell expansion		Differentiation efficiency		CM yield
Cell type	Medium	Cell yield (106 cells/mL)	Cell expansion fold	Sarcomeric actinin	MHC	Cardiomyocyte/hESC
HES-3	SupSB	2.27 ± 0.58	1.65 ± 0.47	14.70% ± 6.30%	13.59% ± 3.40%	0.21 ± 0.08
	SB	1.66 ± 0.48	1.25 ± 0.36	9.25% ± 6.19%	6.17% ± 2.95%	0.07 ± 0.03
H1	SupSB	3.14 ± 0.24	2.35 ± 0.18	26.70% ± 2.09%	25.20% ± 5.41%	0.59 ± 0.01
	SB	1.10 ± 0.19	0.82 ± 0.14	20.23% ± 1.87%	4.76% ± 1.62%	0.04 ± 0.01
Siu1-hiPSC	SupSB	1.22 ± 0.15	0.92 ± 0.11	20.76% ± 4.74%	2.11% ± 0.73%	0.04 ± 0.04
	SB	0.67 ± 0.26	0.51 ± 0.20	16.90% ± 1.95%	1.01% ± 0.20%	0.004 ± 0.001

MHC: Myosin heavy chain. Cultures with SupSB medium were compared against SB medium for cell expansion and differentiation efficiency at the end of culture (day 16). The results were summarized by calculating the cardiomyocyte yield [cardiomyocytes (CM) yield] which takes into account the ratio of cardiomyocytes at day 16 compared to the initial human embryonic stem cells (hESC) seeded (n = 3 to 9).