Dynamic culture improves MSC adhesion on freeze-dried bone as a scaffold for bone engineering

Figure 3 Analysis of cell adhesion (pixels) on bone grafts. Mesenchymal stem cells were co-cultured with freeze-dried bone fragments by two different cultivation methods: dynamic and static co-culture; and in two different groups: cells and bone (CB) and cells, bone and matrix (CBM). After 24 h of culture, there was a significant difference between the two cultivation methods. The dynamic co-culture demonstrated greater cell adhesion on the bone surface than the static co-culture. Three independent experiments were performed for each co-culture system. ^aP < 0.05.