Gene targeting and Calcium handling efficiencies in mouse embryonic stem cell lines

Figure 3 Comparison of agonist-induced changes in [Ca²⁺]_i in mouse R1 and HM-1 embryonic stem cells. The plates were placed into a FlexStation II384 (FLIPR) to monitor cell fluorescence (lEX = 494 nmol/L, lEM = 516 nmol/L, FU, fluorescence units) before and after the addition of various concentrations of agents in high-Ca (1.8 mmol/L) Hank’s medium. A: ATP; B: Bradykinin; C: Histamine; D: Caffeine; E: Thapsigargin.