Gene targeting and Calcium handling efficiencies in mouse embryonic stem cell lines

Table 4 Primer sequences and polymerase chain reaction conditions of genes selected for real time polymerase chain reaction assays

Gene symbol	Gene name	Primer sequence (5' to 3')	Product size (bp)	Annealing T°
S100a4	S100 calcium binding protein A4	For-TCAAGCTGAACAAGACAGAGC	131	56
		Rev-ACTTCATTGTCCCTGTTGCTG
S100a6	S100 calcium binding protein A6	For-TCCACAAGTACTCTGGCAAGG	138	56
		Rev-GGTCCAGATCATCCATCAGC
S100a10	S100 calcium binding protein A10	For-AGCTCTTCCAAGGACTGCTG	138	60
		Rev-TTTGTCAAGTGGTCTTTGTCG
Camkk2	Calcium/calmodulin-dependent protein kinase kinase2, β	For-GTCACACCACGTCTCCATTAC	144	56
		Rev-ACTTTCATTGCGTAATAAGTATTGTC
Tpm1	Tropomyosin 1 α	For-ATGCCCGTGTTCCTTAAAGC	157	59
		Rev-CCCTGACATGGAGAACTGGG
Sparc	Secreted acidic cystein rich glycoprotein	For-ATCCCCATGGAACATTGCAC	122	59
		Rev-TCCTTGTTGATGTCCTGCTCC
Trh	Thyrotropin releasing hormone	For-GGTTCTTCCACGCCTCCTAAG	135	59
		Rev-AACCTTGGAGGATGCGCTG
Lefty1	Left right determination factor 1	For-GCAAACCAAGGACAGAATCCC	108	59
		Rev-TGTTTGCCCAACTTGTGGC
P2rx1	Purinergic receptor P2X, ligand-gated ion channel 1	For-GTCTCCAGGCTTCAACTTC	156	56
		Rev-GAGCCGATGGTAGTCATAGT
P2rx2	Purinergic receptor P2X, ligand-gated ion channel 2	For-GTAGAGCAAGCAGGAGAGA	89	60
		Rev-AGACAAGTCCAGGTCACAG
P2rx3	Purinergic receptor P2X, ligand-gated ion channel 3	For-CCCGCTAAGACCTGAATCT	142	60
		Rev-AGTCCCTGGTATGTGGTAGG
P2rx4	Purinergic receptor P2X, ligand-gated ion channel 4	For-CTGTGTGACGTCATAGTCC	85	60
		Rev-GCTCGTAGTCTTCCACATAC
P2rx5	Purinergic receptor P2X, ligand-gated ion channel 5	For-GCTTTCTTCTGTGACCTG	99	60
		Rev-ATCTTCCTCCTTCTGACC
P2rx6	Purinergic receptor P2X, ligand-gated ion channel 6	For-CCCAAAGACGACTACCAA	86	60
		Rev-CAAACCACTCCTAGGACACT
P2rx7	Purinergic receptor P2X, ligand-gated ion channel 7	For-GGAAGTTAACCGTTCCTG	98	60
		Rev-TGGGCTAGACCTACTTCC
P2ry1	Purinergic receptor P2Y, G-protein coupled 1	For-GGTCTAGCAAGTCTCAACAG	121	60
		Rev-GTAAATTGGCCTCACTCC
P2ry2	Purinergic receptor P2Y, G-protein coupled 2	For-CCAAGCATGGAGAGGAGT	156	60
		Rev-GAATTGCTTGCACCACAG
P2ry4	Purinergic receptor P2Y, G-protein coupled 4	For-ACTAACTGCAGGCAGAGG	170	60
		Rev-CCAGCAAAGAGTACTGAGG
P2ry5	Purinergic receptor P2Y, G-protein coupled 5	For-CATGTACCCGATCACTCTC	136	60
		Rev-GAACCTGGAGTCACTTCTTC
P2ry6	Purinergic receptor P2Y, G-protein coupled 6	For-GAGTTCTGCGTGTGTGTG	172	60
		Rev-GTCAGCCTTTCCTATGCT
P2ry10	Purinergic receptor P2Y, G-protein coupled 10	For-GACATTTGGTATGCAGGCAAG	82	56
		Rev-TGGTCCCTTCCTCTTCCTTAGT
P2ry12	Purinergic receptor P2Y, G-protein coupled 12	For-TGCTGAGGTGCTCAAACTCTAC	84	56
		Rev-GGGTCTCTTCGCTTGGTTC
P2ry13	Purinergic receptor P2Y, G-protein coupled 13	For-AACAGAGCACCAGAAGAGAG	119	60
		Rev-AGGATGCAGATGCTGTTG
P2ry14	Purinergic receptor P2Y, G-protein coupled 14	For-CACAAAGAGTCAGACGGAAGG	96	60
		Rev-ACATTGGCAGCCGAGAGTAG
Bdkrb1	Bradykinin receptor, β 1	For-AGTCGTCCCTGATCTGAA	85	56
		Rev-GTTCAACTCCACCATCCT
Bdkrb2	Bradykinin receptor, β 2	For-CACGCAGATCAGTTCCTAC	99	60
		Rev-ACCTCTCGGGACTTCTTC
Hrh1	Histamine receptor H1	For-GGGCTACATCAACTCCAC	153	60
		Rev-CCCTCTTGGACATCAGAC
Hrh2	Histamine receptor H2	For-CCTCTCCTTCCTCTCTATTC	110	60
		Rev-CCACCAGTCCATATACCTC
Hrh3	Histamine receptor H3	For-ACAGTCAGCAGGAGGGAGAG	135	56
		Rev-TGTCTTCACATTGGCAGAGG
Hrh4	Histamine receptor H4	For-CTTGGAAGAACAGCACGAAC	106	56
		Rev-GAGATGACAGGAAGCAGGAA
Ryr1	Ryanodine receptor 1, skeletal muscle	For-ACTTTGTACCCTGTCCTGTG	132	60
		Rev-CATAGGTCCATCCTTGCTC
Ryr2	Ryanodine receptor 2, cardiac muscle	For-GGATGAATGTCTCACTGTCC	147	60
		Rev-CTTATGTGGCTTCCACTCC
Ryr3	Ryanodine receptor 3	For-CAGGTATCTTGGAGGTCTTG	111	60
		Rev-GCCCATGCTTATCCAGTAG
Itpr1	Inositol 1,4,5-triphosphate receptor 1	For-GGTTGATGACCGTTGTGTTG	121	60
		Rev-GAACTGTTTCTGTGCGGAGT
Itpr2	Inositol 1,4,5-triphosphate receptor 2	For-GTGAGGATGGCATAGAAAG	165	60
		Rev-AGAAGAACAGGAGGTCGTAG
Itpr3	Inositol 1,4,5-triphosphate receptor 3	For-GGCTTCATCAGCACTTTGG	96	60
		Rev-GCAATCTCGGAACTTCTTGG
H2afz	H2A histone family, member Z	For-ACAGCGCAGCCATCCTGGAGTA	202	60
		Rev-TTCCCGATCAGCGATTTGTGGA
Ppia	Peptidylprolyl isomerase A	For-CGCGTCTCCTTCGAGCTGTTTG	150	60
		Rev-TGTAAAGTCACCACCCTGGCACAT