Gene targeting and Calcium handling efficiencies in mouse embryonic stem cell lines

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Stem Cells. Dec 26, 2010; 2(6): 127-140
Published online Dec 26, 2010. doi: 10.4252/wjsc.v2.i6.127

Table 2 Comparative targeting efficiencies of R1 and HM-1 mouse embryonic stem cells

Parameters	Cell line (passage number)
	R1 (p12)					HM-1 (p21)
	Amaxa			BioRad	∑	Amaxa			BioRad	∑
Electroporation program	A23¹	A24¹	A30¹			A23¹	A24¹	A30¹
Cell number	3 × 10⁶			3 × 10⁷			3 × 10⁶			3 × 10⁷
DNA concentration (μg)	5			10		5			10
Number of selected clones	120	192	168	356	836	168	168	24	384	744
Positive clones after 1st PCR screen	3	10	11	15	39	5	4	0	15	24
Percentage (%)	2.5	5.2	6.5	4.2	4.7	2.9	2.4	0	3.9	3.2
Positive clones after 2nd PCR screen	3	10	11	15	39	5	4	0	15	24
Homologous recombination confirmed by Southern blot analysis
Clone number	3	9	10	7	29	4	4	0	5	14
Efficiency (%)	2.5	4.7	6	2	3.5	2.4	2.4	0	1.3	1.9
Transfection efficiency
Transient	No data on Amaxa system			91% ± 6.1%		No data on Amaxa system			75% ± 4.2%
Stable	No data on Amaxa system			34% ± 2.9%		No data on Amaxa system			25% ± 5.3%

¹Amaxa program numbers. PCR: Polymerase chain reaction.

Citation: Mamo S, Kobolak J, Borbíró I, Bíró T, Bock I, Dinnyes A. Gene targeting and Calcium handling efficiencies in mouse embryonic stem cell lines. World J Stem Cells 2010; 2(6): 127-140
URL: https://www.wjgnet.com/1948-0210/full/v2/i6/127.htm
DOI: https://dx.doi.org/10.4252/wjsc.v2.i6.127

Mamo S, Kobolak J, Borbíró I, Bíró T, Bock I, Dinnyes A. Gene targeting and Calcium handling efficiencies in mouse embryonic stem cell lines. World J Stem Cells 2010; 2(6): 127-140 [PMID: 21607130 DOI: 10.4252/wjsc.v2.i6.127]

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