Epigenetic states and expression of imprinted genes in human embryonic stem cells

Table 1 Polymerase chain reaction primer sequences and polymorphisms

Gene	Primer sequence (5'→3')	Size (bp)	SNP	Acc. NoLocation	Ref.
IGF2	F CTTGGACTTTGAGTCAAATTGG	235	G→A	X07868	[18]
	R CCTCCTTTGGTCTTACTGGG			Pos. 820
IPW	F GGGAACTCTTCTGGGAGTGAATGTTATCA	1550	C→T	U12897	[18]
	R GGGAGGTTCATTGCACAGAAATTTGG			Pos. 1670
	Seq. TGGATAGATGCACACAAACAC	868
NESP55 gDNA	F GGCTCCTTGTGCTGTCTGTCTTGTAG	233	T→C	M21741	[18]
	R CCACACAAGTCGGGGTGTAGCTTA			Pos. 299
NESP55 cDNA	F TCGGAATCTGACCACGCGCA	1141
	R CACGAAGATGATGGCAGTCAC
	Seq. CAACCTGAAAGAGGCGATTGAA
PEG10	F TCATTTTCCTGCCTGGTTGC	405	C→T	XM_496907	[19]
	R GGAGCCTCTCATTCACAGC			Pos. 4404
KCNQ1 gDNA	F CACTGCCTGCACTTTGAGCC	282	G→A	AJ006345	[19]
	R GTGAGGAGAAGGGGGTGGTT			Pos. 331010
KCNQ1 cDNA	F GGACCTGGAAGGGGAGACT	282
	R GCGATCCTTGCTCTTTTCT
ATP10A	F AAAGACACCACCGACAGGAA	318	G→C	BC052251	This study
	R ATGCTCATGTCCACTGTGCT			Pos. 4006
TCEB3C	F CCAGAGCTGAGAGAAAGTGC	249	C→G	NM_145653	This study
	R TTTCCTGGCGAGACGATTTG			Pos. 772

gDNA: Genomic DNA; SNP: Single nucleotide polymorphism.