MED12 mutation activates Notch signaling to enhance cancer stemness and suppress CD8+ T cell cytotoxicity in lung cancer

Figure 6 Mediator complex subunit 12 mutation mediated the inhibitory effect of cancer cells on the killing and chemotactic abilities of CD8+ T cells. After co-culture of NCI-H322 and PC9 cells with CD8+ T cells, the following parameters of CD8+ T cells or tumor cells were analyzed. A: Transwell assay was performed to assess the migratory capacity of CD8+ T cells toward tumor cells; B: The expression of interferon-γ, tumor necrosis factor-α, perforin, and granzyme B in CD8+ T cells were measured by enzyme-linked immunosorbent assay; C: Flow cytometry was used to detect the percentage of programmed cell death 1-positive CD8+ T cells; D: The level of lactate dehydrogenase (LDH) in tumor cells was measured by LDH Cytotoxicity Assay Kit to evaluate the cytotoxic activity of CD8+ T cells. Data are presented as mean ± SD. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. All experiments were repeated 3 times. IFN: Interferon; TNF: Tumor necrosis factor.