MED12 mutation activates Notch signaling to enhance cancer stemness and suppress CD8+ T cell cytotoxicity in lung cancer

Figure 2 Mediator complex subunit 12 mutation was involved in the maintenance of cancer stem cells. A: Flow cytometry was used to examine the apoptosis rates of NCI-H322 and PC9 cells; B: Sphere formation assay was used to evaluate the sphere-forming ability of NCI-H322 and PC9 cells; C: Flow cytometry was used to analyze the percentage of ALDH1-positive cells in NCI-H322 and PC9 cells. Data are presented as mean ± SD. ^aP < 0.05, ^bP < 0.01. All experiments were repeated 3 times.