Density gradient centrifugation vs adherence for rat intestinal mesenchymal stem cell isolation

doi:10.4252/wjsc.124197

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World Journal of Stem Cells

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1948-0210

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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Copyright: ©Author(s) 2026. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution-NonCommercial (CC BY-NC 4.0) license. No commercial re-use. See permissions. Published by Baishideng Publishing Group Inc.

World J Stem Cells. Sep 26, 2026; 18(9): 124197
Published online Sep 26, 2026. doi: 10.4252/wjsc.124197

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Figure 2 Morphological comparison of primary cells. A: Conventional adherence (group A): 48 hours, cells migrated out (a1); 7 days, island-like colonies with contaminants (a2); 10 days, target cells and contaminants coexisted (a3); B: Density gradient centrifugation (group B): 48 hours, rapid adhesion (b1); 7 days, homogeneous colonies (b2); 10 days, uniform fibroblast-like morphology (b3). Scale bars: 500 μm (a1, a2, b1, b2) and 200 μm (a3, b3).

Citation: Lai SY, Jiang Y, Yuan R, Wang Z, Wang YC, Zhao ZT, Cai CY, Ruan MY, Zeng YX, Ren B, Wang XF. Density gradient centrifugation vs adherence for rat intestinal mesenchymal stem cell isolation. World J Stem Cells 2026; 18(9): 124197
URL: https://www.wjgnet.com/1948-0210/full/v18/i9/124197.htm
DOI: https://dx.doi.org/10.4252/wjsc.124197

Lai SY, Jiang Y, Yuan R, Wang Z, Wang YC, Zhao ZT, Cai CY, Ruan MY, Zeng YX, Ren B, Wang XF. Density gradient centrifugation vs adherence for rat intestinal mesenchymal stem cell isolation. World J Stem Cells 2026; 18(9): 124197 [DOI: 10.4252/wjsc.124197]

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