Carnitine palmitoyltransferase 1A facilitates the senescence of human dental pulp stem cells by Parkin succinylation-mediated mitophagy

Figure 7 Knockdown of carnitine palmitoyltransferase 1A attenuates D-gal-induced senescence and facilitates mitophagy. A: Carnitine palmitoyltransferase 1A (CPT1A) expression in the dental pulp tissues of mice was measured by quantitative real-time polymerase chain reaction; B-D: CPT1A protein levels in the dental pulp tissues were measured using immunoblotting, and Parkin succinylation levels were detected by immunoblotting after immunoprecipitation; E-L: The levels of aging markers (p53, p21, and p16) (E-H) and mitophagy markers (PINK1, Parkin, and LC3B) (I-L) in the dental pulp tissues of mice were examined using immunoblotting; M: The pathology of dental pulp tissues was evaluated using hematoxylin and eosin staining assay; N: CPT1A and Parkin levels in the dental pulp tissues were detected using immunohistochemistry. n = 6/group; P < 0.001 indicates statistically significant differences. CPT1A: Carnitine palmitoyltransferase 1A; HE: Hematoxylin and eosin.