Estrogen receptor 1 regulates ovarian cancer cell stemness and ferroptosis through modulation of the canonical RhoA/ROCK signaling pathway

Figure 5 Effect of estrogen receptor 1 downregulation on cell function in A2780 cells by inhibiting the Ras homolog gene family member A/Rho-associated coiled-coil containing protein kinase signaling pathway. A2780 cells were transfected with si-NC and si-ESR1, and treated with CN03. A: Cell viability significantly decreased in A2780 cells following transfection with si-ESR1, which was rescued by CN03 treatment; B: Colony formation assay showed reduced cell proliferation in the si-ESR1 group, with restoration upon CN03 introduction; C: 5-ethynyl-2’-deoxyuridine assay revealed decreased cell proliferation in the si-ESR1 group, which was alleviated by CN03 treatment; D: Flow cytometry analysis demonstrated increased apoptosis in A2780 cells post si-ESR1 transfection, with apoptosis inhibition observed in the si-ESR1+ CN03 group; E: Expression levels of B-cell lymphoma-2 associated X and B-cell lymphoma-2 were altered in the si-ESR1 group, with an increase in B-cell lymphoma-2 associated X expression and a decrease in B-cell lymphoma-2 expression, both of which were reversed by CN03 treatment. Data was expressed as mean ± SD (n = 3). ^bP < 0.01 vs si-NC group; ^cP < 0.05 vs si-ESR1 group; ^dP < 0.01 vs si-ESR1 group. ESR1: Estrogen receptor 1; EDU: 5-ethynyl-2’-deoxyuridine assay; Bcl-2: B-cell lymphoma-2; Bax: B-cell lymphoma-2 associated X.