Estrogen receptor 1 regulates ovarian cancer cell stemness and ferroptosis through modulation of the canonical RhoA/ROCK signaling pathway

Figure 4 Effect of estrogen receptor 1 downregulation on the Ras homolog gene family member A/Rho-associated coiled-coil containing protein kinase signaling pathway in A2780 cells. A: Protein-protein interaction network analysis. A2780 cells were transfected with si-NC and si-ESR1, and treated with CN03; B: Real-time polymerase chain reaction analyzed the mRNA levels of RhoA, ROCK1, and ROCK2 in A2780 cells transfected with si-ESR1 with or without CN03; C: Western blot analyzed the protein levels of RhoA, ROCK1, and ROCK2. Data was expressed as mean ± SD (n = 3). ^bP < 0.01 vs si-NC group; ^dP < 0.01 vs si-ESR1 group. ESR1: Estrogen receptor 1; RHOA: Ras homolog gene family member A; ROCK: Rho-associated coiled-coil containing protein kinase.