Estrogen receptor 1 regulates ovarian cancer cell stemness and ferroptosis through modulation of the canonical RhoA/ROCK signaling pathway

Figure 2 Effect of estrogen receptor 1 downregulation on cell function in A2780 cells. A: Real-time polymerase chain reaction analysis revealed a significant decrease in estrogen receptor 1 (ESR1) levels in A2780 cells following transfection with si-ESR1. Then, A2780 cells were transfected with si-NC and si-ESR1; B: Cell viability was notably reduced by Cell Counting Kit-8 assay; C: Cell proliferation was notably reduced by colony formation assay; D: Cell proliferation was notably reduced by 5-ethynyl-2’-deoxyuridine assay; E: Cell apoptosis was increased by flow cytometry; F: Expression levels of B-cell lymphoma-2 associated X were significantly elevated, while B-cell lymphoma-2 expression was decreased. Data was expressed as mean ± SD (n = 3). ^bP < 0.01 vs si-NC group. ESR1: Estrogen receptor 1; EDU: 5-ethynyl-2’-deoxyuridine assay; Bcl-2: B-cell lymphoma-2; Bax: B-cell lymphoma-2 associated X.