Protective effect of hUC-MSCs regulating peritoneal mesothelial cell EMT through cGAS-STING signaling pathway in high glucose-induced peritoneal fibrosis

Figure 1 Activation of the cyclic GMP-AMP synthase-stimulator of interferon genes signaling pathway in in vitro and in vivo models of peritoneal fibrosis. A: 5-ethynyl-2’-deoxyuridine assay was performed to assess the proliferation of HMrSV5 cells under different treatments; B: Quantitative analysis of 5-ethynyl-2’-deoxyuridine assay results; C: Western blot analysis of cyclic GMP-AMP synthase (cGAS) and stimulator of interferon genes (STING) protein expression in HMrSV5 cells; D and E: Quantitative analysis of protein expression in HMrSV5 cells: CGAS (D); STING (E); F: Western blot analysis of cGAS and STING protein expression in peritoneal tissues from different mouse groups; G and H: Quantitative analysis of protein expression in mouse peritoneal tissues: CGAS (G); STING (H). Data are presented as mean ± SD. ^aP < 0.05 vs control. cGAS: Cyclic GMP-AMP synthase; STING: Stimulator of interferon genes; EdU: 5-ethynyl-2’-deoxyuridine.