Dynamic regulation of neural stem cell state transitions by NOTCH1-REST transcriptional axis

Figure 9 RE1-silencing transcription factor inhibition promotes the re-expression of neural stem cell markers in the lesion area. A: Western blot analysis of RE1-silencing transcription factor (REST) and NICD protein expression in each group, with β-actin used as the internal control; B: Immunofluorescence images showing the distribution of Nestin (green) and DAPI (blue) signals in the control and REST inhibition groups. Scale bar = 25 μm. The bar graph on the right shows the quantitative comparison of the proportion of Nestin+ cells (n = 6, three fields per group); C: Immunofluorescence images displaying SOX2 (red) and DAPI (blue) signals in the control and REST inhibition groups. Scale bar = 25 μm. The bar graph on the right shows the quantitative comparison of the proportion of SOX2+ cells (n = 6, three fields per group); D: Co-localization images of Nestin (green) and SOX2 (red) double-positive cells. Scale bar = 25 μm. The bar graph below shows the quantitative comparison of the proportion of Nestin (green) and SOX2 (red) double-positive cells (n = 6, three fields per group). Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by Tukey’s post hoc test, with significance set at P < 0.05. ^aP < 0.05, ^cP < 0.001, ^dP < 0.0001. REST: RE1-silencing transcription factor.