Dynamic regulation of neural stem cell state transitions by NOTCH1-REST transcriptional axis

Figure 8 The organoid model reveals time-dependent regulation of cell states by the Notch-RE1-silencing transcription factor axis in a three-dimensional microenvironment. A: Immunofluorescence images and quantitative analysis of Ki67+ cell proportions following RE1-silencing transcription factor (REST) inhibition and activation treatments. Ki67 is shown in green, DAPI in blue. Scale bar = 50 μm; B: Effects of REST inhibition and REST activation on REST mRNA expression levels (n = 3); C: Correlation analysis between REST expression and Ki67+ cell proportions at different time points under Jagged1 treatment, showing a non-significant negative trend (r = -0.39, P = 0.258). ^cP < 0.001. REST: RE1-silencing transcription factor.