Dynamic regulation of neural stem cell state transitions by NOTCH1-REST transcriptional axis

Figure 7 Single-cell RNA sequencing of organoids reveal RE1-silencing transcription factor activity variations and their association with neurogenesis. A: Volcano plot illustrating the differentially expressed genes between RE1-silencing transcription factor (REST) high-expression and low-expression groups; B: Heatmap of differentially expressed genes between REST high- and low-expression cells. Data were integrated and normalized across developmental stages (DIV20, DIV30, DIV45, DIV60), and visualized using hierarchical clustering; C: Expression profiles of key developmental marker genes (ASCL1, DCX, HES1, NEUROD2, neurogenic locus notch homolog protein 1, REST) across different developmental stages; D: Gene set enrichment analysis enrichment curve for the neurogenesis-related gene set (GO_NEUROGENESIS), calculated based on ranked differential gene expression, showing significant enrichment of neurogenesis pathways in REST low-expression cells. REST: RE1-silencing transcription factor.