Dynamic regulation of neural stem cell state transitions by NOTCH1-REST transcriptional axis

Figure 6 Multiphoton imaging reveals that RE1-silencing transcription factor inhibition accelerates the transition of neural stem cells from dormancy to activation. A: Representative multiphoton images of Nestin+/Ki67+ cells in the NICD overexpression and control groups at 0 hour and 48 hours (Nestin: Green; Ki67: Red; DAPI: Blue), along with bar graphs showing the proportion of activated cells; B: Representative imaging and quantification of activation proportions in the RE1-silencing transcription factor (REST) inhibition and control groups; C: Comparison of activation rates across four treatment groups (vector control, REST inhibition, NICD activation, dual intervention), with statistical significance indicated; D: Time-course activation curves of neural stem cells based on 48 hours continuous imaging data, showing a marked increase within the first 24 hours in the REST inhibition group and a delayed activation in the NICD activation group; E: Kaplan-Meier-style analysis of neural stem cell activation latency, comparing the time distribution for transition from dormancy to activation across treatment groups. ^aP < 0.05, ^bP < 0.01, ^dP < 0.0001. REST: RE1-silencing transcription factor.