Dynamic regulation of neural stem cell state transitions by NOTCH1-REST transcriptional axis

Figure 4 Nuclear localization changes of SOX2 and MCM2 under RE1-silencing transcription factor and Notch signaling regulation. A: Schematic diagram illustrating the regulatory mechanism; B: Quantification of nuclear fluorescence intensity of SOX2 across groups, presented as bar plots; statistical significance was determined by two-tailed t-test; C: Bar graph showing the percentage of MCM2-positive cells in each group; D: Representative high-magnification images demonstrating enhanced nuclear accumulation of SOX2 in the RE1-silencing transcription factor interference group (white arrows); E: Pearson correlation scatter plot with fitted curve illustrating the relationship between SOX2 nuclear intensity and the proportion of MCM2-positive cells; F: Heatmap of SOX2 and MCM2 nuclear localization levels. Colors represent relative expression levels; rows indicate samples, and columns denote gene/protein signal channels. ^aP < 0.05, ^bP < 0.01, ^cP < 0.001. REST: RE1-silencing transcription factor.