Dynamic regulation of neural stem cell state transitions by NOTCH1-REST transcriptional axis

Figure 3 Chromatin accessibility and binding profiles of RE1-silencing transcription factor and neurogenic locus notch homolog protein 1 at representative target gene loci. A-C: The panels respectively display assay for transposase-accessible chromatin using sequencing (ATAC-seq) and chromatin immunoprecipitation sequencing (ChIP-seq) signal distributions at the genomic loci of RE1-silencing transcription factor (REST), DCX, and MKI67. ATAC-seq data were obtained from human neural stem cells under conditions of high REST expression and neurogenic locus notch homolog protein 1 activation. ChIP-seq data include binding signals for REST and neurogenic locus notch homolog protein 1. Signal intensities are presented as normalized read counts (p-2q range). Genomic annotations and scale bars are based on the hg38 reference genome. The horizontal axis represents the genomic coordinate range, with chromosome position and interval length indicated; the scale is shown in kb, and arrows denote the direction of gene transcription. It should be noted that the ATAC-seq and ChIP-seq data for each condition were derived from a single sample (n = 1). The figure therefore presents representative signals from selected genomic regions for exploratory mechanistic analysis. ATAC-seq and ChIP-seq tracks are representative genome-browser views from one biologically independent sample per condition. These data were used for exploratory visualization only, and no statistical differential accessibility or binding analysis was performed. Signal intensity was normalized as reads per genomic content. REST: RE1-silencing transcription factor; ATAC-seq: Assay for transposase-accessible chromatin using sequencing; ChIP-seq: Chromatin immunoprecipitation sequencing.