Dynamic regulation of neural stem cell state transitions by NOTCH1-REST transcriptional axis

Figure 1 Inverse dynamic changes of RE1-silencing transcription factor and neurogenic locus notch homolog protein 1 reveal transcriptional state transitions from quiescence to activation in human neural progenitor cells. A: UMAP plot illustrating single-cell clustering results based on the public dataset GSE104276; B: Neural progenitor cells (NPCs) were subdivided into subpopulations according to RE1-silencing transcription factor expression levels; the term “quiescent-like” denotes a low-proliferative NPC-like state inferred from transcriptional features, not adult NSC deep quiescence; C and D: Pseudotime trajectory of NPCs reconstructed using Monocle3, depicting developmental progression; E: Relative expression patterns of key transcription factors - ASCL1, DCX, HES1, NEUROD, neurogenic locus notch homolog protein 1, and RE1-silencing transcription factor - across the pseudotime trajectory; F: Pseudotime-ordered heatmap showing dynamic gene expression changes along the trajectory. OPCs: Oligodendrocyte precursor cells; NPCs: Neural progenitor cells; REST: RE1-silencing transcription factor.