Platelet-derived mitochondria transfer accelerates fracture healing by promoting osteogenic differentiation and metabolic reprogramming of adipose-derived stem cells

Figure 7 Mitochondria enhance the effect of adipose-derived stem cells on fracture healing by promoting histone lactylation. A: Micro-computed tomography three-dimensional reconstruction image of the fracture location surface; B: Bone microstructure parameters, including bone mineral density, bone volume/total volume, trabecular number, and trabecular thickness were detected using micro-computed tomography; C: Bone histological evaluation was performed using hematoxylin and eosin staining (20 × magnification); D: Cartilage injury was evaluated using saffron O-fast green staining (20 × magnification). n = 6. Data were analyzed by one-way ANOVA followed by Tukey’s post hoc test and are presented as mean ± SD. ASC: Adipose-derived stem cell; BMD: Bone mineral density; BV/TV: Bone volume/total volume; Tb.N: Trabecular number; Tb.Th: Trabecular thickness; 2-DG: 2-deoxy-D-glucose.